bacillus licheniformis v9t14 e coli cft073 s aureus atcc 29213 reduction  (ATCC)

Journal: Scientific Reports

Article Title: Biophysical and transcriptomic characterization of LL-37-derived antimicrobial peptide targeting multidrug-resistant Escherichia coli and ESKAPE pathogens

doi: 10.1038/s41598-025-22890-7

Figure Lengend Snippet: Antimicrobial efficacy of the peptide against various Escherichia coli strains. (A) Minimum inhibitory concentrations (MICs) determined for laboratory E. coli K-12, uropathogenic E. coli (UPEC), and multidrug-resistant (MDR) strain, following CLSI guidelines. Data are shown as mean ± SD from three independent biological replicates, with error bars indicating SD. (B) Time-kill assays showing bactericidal kinetics at four times the MIC concentration, with 90% bacterial reduction achieved within 2 h for all strains. Values represent mean CFU reduction rate ± SD from three independent replicates; error bars indicate SD. (C) Assessment of resistance development after 10 serial passages of each strain in sub-MIC peptide concentrations, indicating no significant increase in MIC and low potential for resistance emergence. MIC values are normalized to passage 1 baseline and presented as mean ± SD from three independent replicates, with error bars indicating SD.

Article Snippet: Bacterial strains used in this study included E. coli K-12 (ATCC® 10798TM, ATCC), uropathogenic E. coli CFT073 (ATCC® 700928TM, ATCC), multidrug-resistant (MDR) E. coli NCTC 13353TM (NCTC), Pseudomonas aeruginosa (ATCC® 27853TM, ATCC), Acinetobacter baumannii (ATCC® 19606TM, ATCC), Klebsiella quasipneumoniae (ATCC® 700603TM, ATCC), Staphylococcus aureus (ATCC® 25923TM, ATCC), and Enterococcus faecium (ATCC® 19434TM, ATCC).

Techniques: Concentration Assay

Journal: Scientific Reports

Article Title: Biophysical and transcriptomic characterization of LL-37-derived antimicrobial peptide targeting multidrug-resistant Escherichia coli and ESKAPE pathogens

doi: 10.1038/s41598-025-22890-7

Figure Lengend Snippet: Mechanism of membrane disruption and transcriptomic response to peptide treatment in E. coli strains. (A) Calcein dye leakage assay demonstrating selective membrane permeabilization of bacterial-mimetic POPE/POPG vesicles by the peptide, with minimal leakage observed in mammalian-mimetic POPC/cholesterol vesicles. Values expressed as % dye leakage relative to 0.1% Triton X-100 (100% release) and shown as mean ± SD from three independent experiments, with error bars indicating SD. (B) RNA sequencing analysis showing upregulation of stress response and efflux pump genes and downregulation of growth-related genes in E. coli K-12, UPEC, and MDR strains after sub-MIC peptide exposure. Data presented as log₂ fold change relative to untreated control; mean ± SE of three biological replicates, with error bars indicating SE. (C) KEGG pathway enrichment analysis highlighting activation of stress response and protein folding pathways alongside repression of DNA replication and cell division pathways across the three E. coli strains. Data represent mean enrichment scores ± SD from three replicates, with error bars indicating SD; enrichment significance adjusted by Benjamini–Hochberg correction (FDR < 0.05).

Article Snippet: Bacterial strains used in this study included E. coli K-12 (ATCC® 10798TM, ATCC), uropathogenic E. coli CFT073 (ATCC® 700928TM, ATCC), multidrug-resistant (MDR) E. coli NCTC 13353TM (NCTC), Pseudomonas aeruginosa (ATCC® 27853TM, ATCC), Acinetobacter baumannii (ATCC® 19606TM, ATCC), Klebsiella quasipneumoniae (ATCC® 700603TM, ATCC), Staphylococcus aureus (ATCC® 25923TM, ATCC), and Enterococcus faecium (ATCC® 19434TM, ATCC).

Techniques: Membrane, Disruption, RNA Sequencing, Control, Activation Assay